WebOct 6, 2024 · The Phospho-YAP (Ser127) (D9W2I) Rabbit mAb Antibodyhas been validated for the following applications: Precipitation, Immunohistochemistry, Immunohistochemistry - fixed, Immunoprecipitation, and Western Blot. Description Phospho-YAP (Ser127) (D9W2I) Rabbit mAb recognizes endogenous levels of YAP protein only when phosphorylated at … WebApr 11, 2024 · After blocking with 5% nonfat milk in TBST, the membranes were incubated with primary antibodies, including anti-Phospho-YAP (Cell Signaling Technology, CST#13008), anti-YAP (Cell Signaling Technology, CST#14074), and anti-S1pr2 antibody (21180-1-AP, Proteintech), followed by HRP-linked secondary antibodies. ... Our results …
Phospho-YAP (Ser127) (D9W2I) Rabbit mAb - Cell …
WebA huge increase of YAP1 protein and YAP phosphorylation at Serine 127 (p-YAP Ser127) in HD over LD was detected. We also observed that DUSP10 overexpressing CRC cell lines, HT29-DUSP10 and HCT116-DUSP10, showed higher levels of YAP1 and p-YAP Ser127 than EV cells in LD, that were further augmented by HD (Figure 2d, Figure S3d). WebHere, we describe a novel approach to detect both the relative amount of total YAP (T-YAP assay) and the phosphorylation of the residue S127 of YAP (S127-P-YAP assay) using a HTRF ®-based method. This easy-to-run method can easily be miniaturized and allows for a high-throughput analysis in 96/384-well plate format, requiring less cellular ... great white spirit animal meaning
Recombinant Anti-YAP1 (phospho S127) antibody …
WebJan 17, 2024 · Phospho (Ser127) View Product On Supplier's Website Request a Quote from Cell Signaling Technology Supplier provided information Immunogen Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser127 of human YAP protein. Validations Biological Strategies … WebImmunoprecipitates from HEK293T cell lysates with indicated antibody were blotted with anti-YAP or anti-NLK antibody. EMBO reports Vol 18 No1 2024 ª 2016 The Authors ... Flag-NLK-WT was transfected and phospho-peaks of YAP at Ser127 when Flag-NLK-KM was transfected (Fig EV2). This finding prompted us to explore whether or not NLK affects … WebAnti-YAP1 (phospho Ser127) antibody (GTX130424) GeneTex Back Primary Antibodies Secondary Antibodies Antibody Panels ELISA Antibody Pairs & Kits Isotype Controls … great white spirit of metal