Ptf1a-creert2
WebØ Ptf1a-201 gene has 2 exons, with the ATG start codon in exon1 and TGA stop codon in exon2. Ø We make Ptf1a-P2A-CreERT2 knockin mice via CRISPR/Cas9 system. Cas9 … WebDownload scientific diagram Pdx1 and Ptf1a transcription factors control early steps of pancreas specification. a Wholemount immunofluorescence for Pdx1 in an E10.5 mouse embryo revealing the ...
Ptf1a-creert2
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WebNov 11, 2024 · The Ptf1a(P48)-Cre mouse allele is a pancreas tissue-specific and acinar cell-specific driver model 32,34,59,60. ... Kras activation in acinar cells using the Mist1-CreERT2, ... WebEgfr f/f ;Ptf1a FlpO/+ ;FSF-Rosa26 CAG-CreERT2 mice allow conditional deletion of EGFR in pancreatic epithelial cells in a timely manner by the administration of tamoxifen (Wen et …
WebJan 5, 2016 · To inactivate Bptf to analyse its role in c-MYC-driven pancreatic tumorigenesis, we administered 25 mg of tamoxifen (Sigma-Aldrich, T-5648) by gavage over the course of 1 week to 5–7-week-old Bptf lox/lox;Ptf1a-CreERT2 +/KI;Ela1-Myc mice and their corresponding controls. Mice were screened for pancreatic tumours once a week using a … WebThe results confirm the presence of multipotent ptf1a‐expressing progenitor cells in developing zebrafish pancreas, with reduced pTF1a dosage promoting greater contributions towards non‐acinar lineages and underscoring the central role of p TF1a in foregut tissue specification. Background: Pancreas development in zebrafish shares many features with …
WebPtf1a is preferably assembled into a transcription trimeric complex PTF1 with an E protein and Rbpj (or Rbpjl). In pancreatic development, Ptf1a is indispensable in controlling the … WebMar 1, 2024 · Corresponding mouse lineage-tracing studies using Cpa1 CreERT2 and Ptf1a CreERTM also revealed a shift from multipotency to acinar-specific unipotency after secondary transition (Zhou et al., 2007), while multipotency was found to be (re)activated in Ptf1a + cells by injury, e.g. partial duct ligation (Pan et al., 2013).
WebTo delineate the lineage potential of ptf1a-expressing cells, we generated ptf1a:creERT2 transgenic fish and performed genetic-inducible lineage tracing in developmental, regenerating, and ptf1a-deficient zebrafish pancreas. Results: In addition to their contribution to the acinar cell lineage, ptf1a-expressing cells give rise to both ...
Webcerebellar cortex. The data in Figure S1 also demonstrate that Ptf1a+ cells in the PWM, which we show respond to Shh, contribute exclusively to GABAergic interneurons. Figure S2. Figure S2 provides important results from our Gli1 fate-mapping studies that are essential to our interpretation of the data presented in Figure 2. how much are scratch off lottery ticketsWebNumbers of Ptf1a + Sox9 + cells decreased rapidly as organogenesis progressed. (P) Schematic, lineage potency of Ptf1a + Sox9 + Hnf1 + tip MPC during the 2° transition. Scale bars: 50 mm. from ... photon turnoverWebThe results confirm the presence of multipotent ptf1a‐expressing progenitor cells in developing zebrafish pancreas, with reduced pTF1a dosage promoting greater … photon treatmentWebTgBAC(ptf1a:Cre-ERT2) ID ZDB-TGCONSTRCT-151008-6 Name TgBAC(ptf1a:Cre-ERT2) Previous Names. tg(ptf1a:creERT2) Type engineered_plasmid Regulatory Regions. ptf1a ; … how much are sea levels predicted to riseWebPtf1a-CreERT2, Ptf1a em1(CreERT2-WPRE-pA)Smoc Gene: Ptf1a Location: Chr2:19450474-19452312 bp, + strand Genetic Position: Chr2, 13.37 cM, cytoband A2 how much are scrubshttp://www.informatics.jax.org/allele/MGI:6758049 photon ventures inchow much are screenwriters paid